Fig. 3

BRCA1 binds to PDGFRβ locus and represses transcription of PDGFRβ and EMT-associated genes. a, b HCC1937 (a) and MDA-MB231 (b) cells were transfected with pBabe-empty (Empty), pBabe-HA-BRCA1 (BRCA1-a), or pBabe-Myc-BRCA1 (BRCA1-b). Expression of genes indicated in the cells were determined by western blot and qRT-PCR 48 h after transfection. c Diagram showing the location of putative BRCA1 binding sites (red bars) in the human PDGFRβ gene. + 1, transcription start site. d ChIP analysis of endogenous BRCA1 binding to the PDGFRβ locus in T47D cells. The results were normalized to the amount of input and compared with the IgG-negative controls. Data are represented as mean ± SD. e ChIP analysis of exogenous BRCA1 binding to the PDGFRβ locus in HCC1937 cells transfected with Babe-empty (Empty) or pBabe-HA-BRCA1 (BRCA1). The results were normalized to the amount of Input and compared with the empty controls. Data are represented as mean ± SD