Circulating 27-hydroxycholesterol and breast cancer tissue expression of CYP27A1, CYP7B1, LXR-β, and ERβ: results from the EPIC-Heidelberg cohort

Table 3 Prediagnosis circulating 27HC and breast cancer risk by breast tumor expression of select IHC markers: EPIC-Heidelberg nested case-control study

			Menopausal status at blood collection
		All women	Premenopausal	Perimenopausal	Postmenopausal	p_het^b
All women	Cases/controls	286/1029	86/315	54/195	146/519
All women	OR (95% CI)^a	0.84 (0.54,1.30)	1.23 (0.53,2.87)	1.78 (0.61,5.19)	0.58 (0.31,1.08)	0.15
CYP27A1
Negative	Cases/controls	201/1029	61/315	37/195	103/519
Negative	OR (95% CI)^a	0.81 (0.48,1.35)	1.08 (0.39,2.96)	1.68 (0.45,6.27)	0.54 (0.26,1.11)	0.25
Positive	Cases/controls	72/1029	22/315	14/195	36/519
Positive	OR (95% CI)^a	1.31 (0.60,2.89)	2.01 (0.42,9.58)	2.30 (0.26,20.48)	1.07 (0.35,3.26)	0.79
p_het		0.32	0.51	0.73	0.32
CYP7B1
Negative	Cases/controls	156/1029	46/315	30/195	80/519
Negative	OR (95% CI)^a	0.90 (0.51,1.59)	1.54 (0.49,4.84)	5.79 (1.22,27.4)	0.58 (0.26,1.29)	0.15
Positive	Cases/controls	86/1029	29/315	19/195	38/519
Positive	OR (95% CI)^a	0.74 (0.35,1.56)	1.10 (0.26,4.64)	0.53 (0.08,3.37)	0.37 (0.12,1.11)	0.17
p_het		0.60	0.82	0.22	0.61
LXR-β
Negative	Cases/controls	122/1029	38/315	22/195	62/519
Negative	OR (95% CI)^a	0.85 (0.46,1.59)	1.15 (0.33,4.03)	16.64 (2.54,108.87)	0.44 (0.18,1.09)	0.02
Positive	Cases/controls	164/1029	48/315	32/195	84/519
Positive	OR (95% CI)^a	0.87 (0.50,1.53)	1.31 (0.43,4.01)	0.76 (0.18,3.14)	0.72 (0.33,1.58)	0.69
p_het		0.91	0.70	0.02	0.34
ERβ
Negative	Cases/controls	83/1029	24/315	18/195	41/519
Negative	OR (95% CI)^a	0.73 (0.35,1.53)	1.24 (0.29,5.37)	6.66 (0.97,45.7)	0.31 (0.10, 0.92)	0.06
Positive	Cases/controls	203/1029	62/315	36/195	105/519
Positive	OR (95% CI)^a	0.86 (0.52,1.44)	1.27 (0.46,3.5)	0.93 (0.22,3.8)	0.72 (0.36,1.47)	0.66
p_het^c		0.67	0.96	0.17	0.12

Missing: CYP7B1 = 44, CYP27A1 = 14. Also, 8 women have missing for date at blood draw and fasting status at blood collection: 1 case and 7 controls
^aOdds ratios (ORs) per one-unit increase in log2 transformed 27HC; all ORs adjusted for matching factors plus age at first menstrual period, BMI, age at first full term pregnancy, number of full term pregnancies, and matching factors, namely, age, time, and date at blood draw, menopausal status, exogenous hormone use, and fasting status at blood collection, and additionally menstrual phase for premenopausal women
^bComparing pre-, peri-, postmenopausal
^cComparing tumor expression

ISSN: 1465-542X