Table 1 Immunohistochemistry (IHC) of breast tumor marker expression distribution: EPIC-Heidelberg nested case-control study

CYP27A1

Abcam

Positive = moderate or strong (2 or 3) intensity in cytoplasm; otherwise negative

–

(EPR7529)

1:50

+

CYP7B1

Abcam (ab19043)

1:500

Positive = moderate or strong (2 or 3) intensity in cytoplasm of ≥ 10% of cells; otherwise negative

–

+

LXR-β

Abcam (ab24361)

1:25

Positive = moderate or strong (2 or 3) intensity in nucleus of ≥ 10% of cells; otherwise negative

–

+

ERβ

Serotec (PPG5/10)

1:500

Positive = moderate or strong intensity (2 or 3) nuclear staining in > 50% tumor cells; otherwise negative

–

+