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Fig. 4 | Breast Cancer Research

Fig. 4

From: p66ShcA functions as a contextual promoter of breast cancer metastasis

Fig. 4

Non-mitochondrial functions of p66ShcA support breast cancer cell migration. a Average migration speed of the indicated cell lines was determined by live-cell imaging. The data is representative of average speed (μm/h) ± SEM from three independent experiments. The number of cells analyzed per cell line is indicated in parentheses. b Frequency distribution of migration speeds from panel A. c Live cell migration tracks of the indicated cell lines on fibronectin-coated plates. Each line represents the migration path of a single cell over a 6-h period (parental: n = 102; p66-CR (VC): n = 109; p66-CR (WT): n = 118; p66-CR (S36A): n = 98). The starting point of each cell was superimposed onto the origin (0, 0). Tracks were color-coded based on cell speed (black, < 20 μm/h; red, 20–30 μm/h; yellow 30–40 μm/h; green > 40 μm/h). Data represents tracks from three independent experiments. d Boyden chamber assays to determine the migratory properties of the indicated cell lines. The data is shown as fold change in cell migration relative to the parental 4T1-537 cell line ± SEM and is representative of 9 wells over three independent experiments. Representative images are shown. For panels a and d, statistical analysis was performed using a one-way ANOVA with a Tukey’s multiple comparisons test (*P < 0.05; ***P < 0.001; ****P < 0.0001; δδδP < 0.001, and δδδP < 0.0001 represents VC versus p66ShcAWT or VC versus p66ShcAS36A comparisons)

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