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Fig. 1 | Breast Cancer Research

Fig. 1

From: p66ShcA functions as a contextual promoter of breast cancer metastasis

Fig. 1

p66ShcA expression is not sufficient to enhance triple-negative breast cancer lung metastasis. a Immunoblot analysis of ShcA in vector control (VC) and p66ShcA-overexpressing parental 4T1 breast cancer cells. An immunoblot for α-Tubulin served as a loading control. b Mammary fat pad (MFP) injections of VC and p66ShcA-overexpressing parental 4T1 cells. The data is shown as average tumor volume (mm3) ± SEM (n = 13 tumors/group). c Cleaved caspase-3 IHC of VC and p66ShcA-overexpressing 4T1 mammary tumors. The data is shown as average percentage of positive cells ±SEM (n = 8 tumors/group). Representative images are shown. d Ki67 IHC staining of VC and p66ShcA-overexpressing 4T1 mammary tumors. The data is shown as the average percentage of positive cells ±SEM (n = 8 tumors/group). Representative images are shown. e Metastatic tumor burden in the lungs of mice bearing VC- and p66ShcA-overexpressing 4T1 mammary tumors. Mammary tumors were resected at 500 mm3, and the development of lung metastases was quantified 14 days later. The data is shown as the average percentage of lung metastasis area to lung tissue area ± SEM (n = 8 tumors/group). Representative H&E images are shown. Statistical analysis was performed using a two-tailed unpaired Student’s t test (*P < 0.05; **P < 0.01)

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