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Fig. 3 | Breast Cancer Research

Fig. 3

From: High B3GALT5 expression confers poor clinical outcome and contributes to tumor progression and metastasis in breast cancer

Fig. 3

Effects of B3GALT5 knockdown on cell migration and invasion. a mRNA expression of B3GALT5 in AS-B634 cells transfected with control siRNA (si-Ctrl) or B3GALT5 siRNA (si-B3GALT5-1 and si-B3GALT5-2) as determined by qRT-PCR. ****P < 0.0001 compared to the si-Ctrl control by t test. b, c Expression of B3GALT5 downstream target SSEA-3 detected by flow cytometry. d, f Representative pictures of transwell cell migration and invasion assays of AS-B634. Scale bar 100 μm. e, g Quantitation of migrated or invaded cells per well. Data were shown as mean ± SD for triplicates. **** P < 0.0001, ***P < 0.001, **P < 0.01 by t test

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