Fig. 5
From: Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer
CTSV mediates GATA3 degradation through Akt and GSK-3β signalling. a, b Western blotting analysis of pThr 308 Akt, pSer473 Akt, Akt, pSer9 GSK-3β, GSK-3β pSer308 GATA3 and GATA3 expression in MCF-7 and ZR-75-1 shCTSV cell line models. Restoration of CTSV expression in sh1 cells was enabled by the generation and transfection of an shRNA-resistant rescue construct (rCTSV). c, d Western blotting analysis of GATA3 expression following transfection of STYX (Flag-tagged) or pCMV empty vector (EV) into MCF-7 or ZR-75-1 cells. e, f Western blotting analysis of GATA3 expression in MCF-7 and ZR-75-1 cells following treatment with Buparlisib (250 μM, 6 h). GAPDH was used as an internal loading control for all Western blotting analysis