Fig. 2

Evaluation of ABT-263/navitoclax+ABT-414 treatment responses. Seven EGFR-expressing PDX models were treated with ABT-263+ABT-414 [combo] or the vehicles for both agents [vehicle] according to the dose and schedule presented in Supplemental Figure 1a. Fourteen days post-treatment, significant tumor growth inhibition and regressions were observed in five of seven PDX models: HCI-001, HCI-002, HCI-004, HCI-010, and HCI-019 (a). The graphs present tumor growth as a percent of pre-treatment tumor volumes for each tumor (n = 3–6 per group). Each symbol represents a tumor. Each line represents the mean. Note that consistent and substantial regressions are restricted to HCI-010 tumors. p values < 0.05 (*), < 0.01 (**), < 0.001 (***), and < 0.0001 (****) are indicated. p values > 0.05 = NS. p values, determined by Welch’s one-tailed test, are presented for vehicle versus combo comparisons in Supplemental Table 2a. Representative H&E images for vehicle- (b) and combo-treated (c) tumors are shown to highlight treatment-associated pathological responses within HCI-002, HCI-010, and HCI-015 tumors. Note that HCI-002-treated tumors were characterized by regions of multi-focal cellular dropout; HCI-010-treated tumors were characterized by a desmoplastic stroma; HCI-015-treated tumors displayed reduced tumor cell content (→ ←) associated with expanded areas of necrosis. Scale bar, ~ 200 μm