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Table 3 Peptide candidates selected for the MRM assay

From: Shotgun proteomics coupled to nanoparticle-based biomarker enrichment reveals a novel panel of extracellular matrix proteins as candidate serum protein biomarkers for early-stage breast cancer detection

Gene Peptide sequence Precursor m/z Precursor charge
FERMT3 VFVGEEDPEAESVTLR 888.93 2
VVLAGGVAPALFR 635.38 2
ACTG1 GYSFTTTAER 566.77 2
ACTG1/POTEF AGFAGDDAPR 488.73 2
AVFPSIVGRPR 599.86 2
ACTN (ACTN1/ACTN4) VGWEQLLTTIAR 693.89 2
LASDLLEWIR 608.34 2
GP1BB LSLTDPLVAER 607.34 2
RAP1 (RAP1A/RAP1B) LVVLGSGGVGK 493.31 2
SKINVNEIFYDLVR 570.65 3
SALTVQFVQGIFVEK 833.46 2
TUBA EIIDLVLDR 543.31 2
LISQIVSSITASLR 744.44 2
TUBA VGINYQPPTVVPGGDLAK 913.00 2
TUBB1 GASALQLER 472.76 2
EVDQQLLSVQTR 708.38 2
TUBB (TUBB1/TUBB3/TUBB6) FPGQLNADLR 565.80 2
ITGB3 SKVELEVR 480.28 2
PFN1 STGGAPTFNVTVTK 690.36 2
TFVNITPAEVGVLVGK 822.47 2
CFL1 LGGSAVISLEGKPL 670.89 2
BMP1 LNGSITSPGWPK 628.84 2
LTF DGAGDVAFIR 510.76 2
ITGA2B VAIVVGAPR 441.28 2
VYLFLQPR 518.30 2
THBS1 SITLFVQEDR 604.32 2
GFLLLASLR 495.31 2
FLNA; FLN1 ANLPQSFQVDTSK 717.86 2
YGGQPVPNFPSK 645.83 2
SPFSVAVSPSLDLSK 767.41 2
MYH9 ALELDSNLYR 597.31 2
HPSE FLILLGSPK 494.32 2
TDFLIFDPK 548.29 2
AHSG HTFMGVVSLGSPSGEVSHPR 699.68 3
TLN1 LAQAAQSSVATITR 708.89 2
ILAQATSDLVNAIK 728.92 2
GLAGAVSELLR 543.32 2
TLN2 VMVTNVTSLLK 610.85 2
SIAAATSALVK 516.31 2
MST1 VVGGHPGNSPWTVSLR 831.94 2
LIMS2 VIEGDVVSALNK 622.35 2
  1. Peptides were selected using the following criteria: highest number of identified peptides and intensities from discovery dataset, presence of at least 3 high abundant − b and/or − y product ions, highest ESP scores and scored transitions in the PeptideAtlas, no Met residues, and + 2 or + 3 charge state precursor ions. The protein isoforms are described in parenthesis