Skip to main content
Fig. 6 | Breast Cancer Research

Fig. 6

From: Immune phenotype of patients with stage IV metastatic inflammatory breast cancer

Fig. 6

Correlations between PD-1 and PD-L1 expression in tumor samples and infiltrating T and B cells by IHC. The eight tumor samples analyzed in Fig. 5 were stained for CD3 to mark infiltrating T cells, CD20 to mark infiltrating B cells, and hematoxylin. The sample from patient 10 had limited available tissue, so this was excluded from the CD3 staining analysis. a CD3 and CD20 staining in brown is shown in samples from a representative patient. Magnification is × 100 in each image and bars designate 200 μm. Percentages of CD3+ or CD20+ TIL were assessed by a pathologist as percentage of positive cells relative to total nucleated cells in tumor and immediately peri-tumoral areas. Percentage scores for staining of TIL (x-axis) were correlated to scores for PD-1 and PD-L1 (y-axis) from the same tumor biopsies in individual patients are shown as: b % CD20+ TIL vs. PD-1, c % CD20+ TIL vs. PD-L1, and d % CD3+ TIL vs. PD-1. Numbers next to square icons designate the number of multiple superimposed data points at that position

Back to article page