Fig. 2
From: Mutant p53 drives the loss of heterozygosity by the upregulation of Nek2 in breast cancer cells
Mutp53 suppresses cell cycle checkpoint following γ-irradiation. a Aberrant cell cycle checkpoint following γ-irradiation in p53H/−;ErbB2 cells. Bar graphs showing cell cycle analysis of p53+/+;ErbB2, p53H/+;ErbB2, p53−/+;ErbB2, and p53H/−;ErbB2 cell lines irradiated (gray bars) or not (black bars). n = 3 independent experiments per genotype (one cell line per genotype except for p53+/+ and p53 H/− where 2 different cell lines derived from different tumors and result per genotype was averaged). b Western blot analysis of p21 level before and 24 h after γ-irradiation (0.1 Gy) in mouse ErbB2 mammary epithelial tumor cell lines with different p53 status. α-Tubulin is loading control. c Bar graph showing relative mRNA expression level of CycE before and 24 h after γ-irradiation in ErbB2 mammary epithelial tumor cell lines with different p53 status. n = 3 independent experiments per cell line per genotype. d Bar graph showing percent viability before and 24 h after γ-irradiation in ErbB2 mammary epithelial tumor cell lines with different p53 status. n = 3 independent experiments per cell line per genotype. e Restoration of cell cycle checkpoint 24 h post γ-irradiation p53-null cells. Bar graphs showing cell cycle analysis of p53H/−;ErbB2, p53H/+;ErbB2, p53−/+;ErbB2 following CRISPR/Cas9 p53 deletion (p53CC9) and in p53−/−;ErbB2 cell lines irradiated (gray bars) or not (black bars). n = 3 independent experiments per genotype. f Bar graphs showing mitotic index in different cell lines irradiated (gray bars) or not (black bars) (result for each irradiated genotype was compared to its own control). n = 3 independent experiments per genotype. Where applicable *p < 0.05; **p < 0.01; ***p < 0.001. Error bars represent ± SD