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Fig. 1 | Breast Cancer Research

Fig. 1

From: Telomere lengths in women treated for breast cancer show associations with chemotherapy, pain symptoms, and cognitive domain measures: a longitudinal study

Fig. 1

Fluorescence in situ hybridization (FISH) chromosome-specific telomere images. ad The steps used for the chromosome-specific telomere assay. Individual chromosomes from each metaphase cell were evaluated based on information gained from their DAPI counterstaining appearance (a), which was used to generate a reverse DAPI banding image (b). The intensity/size of the telomere probe signal (c) was also evaluated, with a propidium iodide (PI) stain being used to delineate the chromosomal boundaries (d). e Based on the collective information gained from the reverse DAPI, FITC labeled, merged, and propidium iodide (PI) images (shown from left to right), ratio profiles were calculated for each chromosome. In image e, a representative chromosome 3 shows a short arm telomere (top of chromosome) that is larger (fluorescence intensity value of 6.63) than the long arm telomere (bottom of chromosome; value of 4.63)

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