Table 1 Antibodies used in this study
Antibody | Dilution | Supplier | |
|---|---|---|---|
Antigen | |||
Vimentin^,# | Mouse Monoclonal IgG (V9) | 1:750 | Dako, Australia |
E-cadherin^,# | Mouse Monoclonal IgG (36) | 1:12,500 | BD Transduction Laboratories, USA |
N-cadherin (A-CAM) | Mouse Monoclonal IgG (GC-4) | 1:2000 | Sigma-Aldrich, Australia |
TWIST^ | Mouse/ 2C1a | 1:100 | Abcam, England |
Beta-catenin^ | Mouse monoclonal, clone 14 | 1:500 | BD Biosciences, Australia |
P120^ | Mouse monoclonal 98/pp120 | 1:200 | BD Biosciences, Australia |
CD24^ | Mouse monoclonal SN3b | 1:50 | Thermo Fisher Scientific, Australia |
CD44^ | Mouse monoclonal 156-3C11 | 1:75 | Abcam, England |
Ki-67^ | Mouse monoclonal MIB-1 | 1:100 | Dako, Australia |
CA-IX^ | Rabbit monoclonal EP161 | 1:100 | Cell Marque (Sigma Aldrich), USA |
ITGB1# | Mouse Monoclonal IgG | 1:5000 | Chemicon International (Fisher Scientific), USA |
ITGA2# | Rabbit Polyclonal | 1:1000 | Chemicon International (Fisher Scientific), USA |
Integrin-Linked Kinase (ILK)# | Rabbit monoclonal IgG | 1:4500 | Cell Signaling Technologies (Danvers, MA,USA) |
Pan-Actin, Ab-5# | Mouse Monoclonal IgG | 1:10,000 | Neomarkers (Invitrogen), USA |
Secondary antigen | |||
Biotinylated Immunoglobulin^,# | Polyclonal Rabbit Anti-Mouse | 1:200 | Dako, Australia |
IgG-HRP^,# | Goat Anti-Mouse | 1:20,000 | Dako, Australia |
IgG-HRP^,# | Goat Anti-Rabbit | 1:20,000 | Dako, Australia |