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Table 1 Antibodies used in this study

From: Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer—insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system

  Antibody Dilution Supplier
Antigen
 Vimentin^,# Mouse Monoclonal IgG (V9) 1:750 Dako, Australia
 E-cadherin^,# Mouse Monoclonal IgG (36) 1:12,500 BD Transduction Laboratories, USA
 N-cadherin (A-CAM) Mouse Monoclonal IgG (GC-4) 1:2000 Sigma-Aldrich, Australia
 TWIST^ Mouse/ 2C1a 1:100 Abcam, England
 Beta-catenin^ Mouse monoclonal, clone 14 1:500 BD Biosciences, Australia
 P120^ Mouse monoclonal 98/pp120 1:200 BD Biosciences, Australia
 CD24^ Mouse monoclonal SN3b 1:50 Thermo Fisher Scientific, Australia
 CD44^ Mouse monoclonal 156-3C11 1:75 Abcam, England
 Ki-67^ Mouse monoclonal MIB-1 1:100 Dako, Australia
 CA-IX^ Rabbit monoclonal
EP161
1:100 Cell Marque (Sigma Aldrich), USA
 ITGB1# Mouse Monoclonal IgG 1:5000 Chemicon International (Fisher Scientific), USA
 ITGA2# Rabbit Polyclonal 1:1000 Chemicon International (Fisher Scientific), USA
 Integrin-Linked Kinase (ILK)# Rabbit monoclonal IgG 1:4500 Cell Signaling Technologies (Danvers, MA,USA)
 Pan-Actin, Ab-5# Mouse Monoclonal IgG 1:10,000 Neomarkers (Invitrogen), USA
Secondary antigen
 Biotinylated Immunoglobulin^,# Polyclonal Rabbit Anti-Mouse 1:200 Dako, Australia
 IgG-HRP^,# Goat Anti-Mouse 1:20,000 Dako, Australia
 IgG-HRP^,# Goat Anti-Rabbit 1:20,000 Dako, Australia
  1. ^Antibody used for IHC
  2. #Antibody used for WB