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Fig. 8 | Breast Cancer Research

Fig. 8

From: Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer—insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system

Fig. 8

Migration properties of PMC42-ET cells following siRNA knockdown of ITGB1, ITGA2 and ILK without and with stimulation by EGF. Effect on migration was assessed using the a Boyden Chamber and b scratch-wound assays. Data are expressed as percentage of control and represent the mean ± SD from 3 biological replicates. c Effect of ILK inhibitor QLT0267 (used at 6.25 μM, in DMSO) on PMC42-ET cell migration in the Boyden Chamber assay. Results represent average cell counts from of 5 random high-power fields from three independent experiments, error bars represent standard deviation. For a, statistical significance (*p < 0.05, comparison to control siRNA) was determined using two-way ANOVA, with Dunnett’s multiple comparison test. For b, statistical significance was determined using two-way ANOVA followed by Holm-Sidak’s multiple comparisons test. For c, statistical significance (***p < 0.0001) was determined using unpaired two-tailed t test with Welch’s correction

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Breast Cancer Research

ISSN: 1465-542X

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