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Fig. 6 | Breast Cancer Research

Fig. 6

From: Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer—insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system

Fig. 6

a Western blots showing ITGB1 (130 kDa), ITGA2 (160 kDa) and ILK (50 kDa) versus pan-actin (ACTN, 45 kDa) protein expression of siRNA transfected PMC42-ET cells; siRNA knockdown did not affect EMT of PMC42-ET cells in response to 10 ng/ml EGF treatment (+EGF) after 72 h as shown by b. western blotting for vimentin, (53 kDa), NCAD (100 kDa) and E-cadherin (120 kDa); numbers shown below the Western blots in a and b represent respective protein expression after normalisation to pan actin, relative to cells treated with control siRNA; TF: Transfection. c Phase contrast cellular morphology of cells from which protein was extracted for Western blots shown in b. Scale bar, 100 μm. The results of experiments shown are representative of three independent experiments

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Breast Cancer Research

ISSN: 1465-542X

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