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Table 1 Abundant proteins identified uniquely in MDA-MB231 or MCF10DCIS.com EVs purified from cell culture supernatants

From: Extracellular vesicles from young women’s breast cancer patients drive increased invasion of non-malignant cells via the Focal Adhesion Kinase pathway: a proteomic approach

MDA-MB231 EVs MCF10DCIS.com EVs
Protein name No. of spectral matchesA Protein name No. of spectral matchesA
Complement C4-B 140.2 Protocadherin fat 2 264.6
EGF-like repeat and discoidin I-like protein 3 101.7 Laminin subunit beta-3 238.6
Vitronectin 101.2 Chondroitin sulfate proteoglycan 2 200.7
Annexin A6 88.5 CD109 antigen 196.8
NUMA1 variant protein 88.3 Retinoic acid receptor responder protein 1 189.0
Filamin-C 50.3 Prostaglandin F2 receptor negative regulator 160.1
Thioredoxin reductase 1 47.5 Laminin, alpha 4 147.3
Collagen, type V, alpha 1 44.4 Syndecan-1 139.9
Proteasome subunit beta type-4 39.4 Protocadherin fat 1 110.0
Filamin-A 38.2 Stromal cell derived factor 4 108.3
U5 small nuclear ribonucleoprotein 37.9 Epithelial cell adhesion molecule 105.3
Early endosome antigen 1 37.6 Protein S100-A14 97.3
Ectonucleotide pyrophosphatase 36.2 SPARC related modular calcium binding 1 83.9
Proteasome subunit alpha type-1 32.4 Fibulin-1 68.9
Interleukin enhancer-binding factor 2 30.8 Suppressor of tumorigenicity 14 protein 65.6
60S ribosomal protein L12 28.7 Catenin, beta 1 59.4
Ubiquitin-like protein ISG15 27.8 Serpin peptidase inhibitor, clade E 56.1
Aspartate-tRNA ligase 25.6 Follistatin 48.0
Proteasome subunit beta type 7 24.5 Collagen alpha-1 45.2
Serum paraoxonase/arylesterase 1 23.1 Claudin 45.1
26S proteasome non-ATPase subunit 6 22.1 Amphiregulin 44.0
Lysosomal-associated membrane protein 1 21.5 Lipolysis-stimulated lipoprotein receptor 43.8
Ferritin light chain 20.8 Fascin 42.5
26S protease subunit 8 20.6 Plakophilin-3 41.5
60S ribosomal protein L27 19.8 Isoform 4 of scavenger receptor class B1 40.5
SARS protein 19.3 Solute carrier 16, member 1 37.1
  1. Of the 1109 proteins identified in MCF10DCIS.com EVs and the 1032 proteins identified in MDA-MB231 EVs, 632 proteins were shared between the two cell lines
  2. ASpectral matches are the averages of two independent EV preparations