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Fig. 3 | Breast Cancer Research

Fig. 3

From: Extracellular vesicles from young women’s breast cancer patients drive increased invasion of non-malignant cells via the Focal Adhesion Kinase pathway: a proteomic approach

Fig. 3

EVs from YWBC patients have a unique proteomic signature. EVs from 20 YWBC patients and 10 healthy donors were purified by size-exclusion chromatography and analyzed by mass spectrometry. The identified proteins were analyzed using MetaboAnalyst 3.0 software. a Of the 583 proteins identified, EVs from YWBC patients contain 94 unique proteins compared to EVs from healthy donors. b Multivariate analysis was performed using the partial least squares discriminant analysis method. Variables were sorted into components according to their ability to discriminate between YWBC patients and healthy donors (HD). The 3D plot of component 1 (A = 0.83, R2 = 0.82, Q2 = 0.50), component 2 (A = 0.97, R2 = 0.82, Q2 = 0.71), and component 3 (A = 0.96, R2 = 0.97, Q2 = 0.64) is shown, in which the number of spectral matches for proteins in these groups distinguishes YWBC (blue) from HD (black). A volcano plot analysis was performed using MetaboAnalyst 3.0, comparing the proteomic differences between EVs from YWBC patients and HD (c) or EVs with functional activity in Fig. 2 and those with no effect (d). Fold change values are represented on the x-axis, and t test p values are represented on the y-axis (see Additional file 4 for specifics). Proteins of interest for breast cancer are labeled

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