Fig. 4
From: CRIPTO antagonist ALK4L75A-Fc inhibits breast cancer cell plasticity and adaptation to stress
The CRIPTO blocking agent, ALK4L75A-Fc, reduces cancer stem cell properties in stressed breast cancer cells. a Stress and ALK4L75A-Fc associated changes in Aldefluor markers. b, c Effects of ALK4L75A-Fc on CD44 expression in thapsigargin- or 2-DG-treated MDA-MB-231 (b) and MDA-MB-468 cells (c). *Student’s t test, p < 0.05. d Changes in suspension colony frequency and growth of LVS14-Mock- and LVS14-ALKL75A-Fc-transduced MDA-MB-468 cells following stress. Duplicate wells were examined, and the experiment was carried out twice. e Quantification of suspension colony growth from the experiment shown in d and using multiple images. *Fisher’s exact test, p < 0.05. f Serial passage rates of MDA-MB-468 cells (in triplicate) induced to express ALKL75A-Fc. *Kruskal-Wallis, p = 0.0152