Fig. 2
From: CRIPTO antagonist ALK4L75A-Fc inhibits breast cancer cell plasticity and adaptation to stress
Stress response of the CRIPTO signaling pathway in breast cancer cell lines. a RT-PCR for TDGF1 (CRIPTO) in MDA-MB-231 under serum starvation. b Confirmation of TDGF1 sequence of the PCR product from a. c Western blot indicating that ALK4L75A-Fc blocks starvation-induced AKT activation in MDA-MB-231 cells. d AKT activation in MDA-MB-231 cells harboring a doxycycline-inducible shCRIPTO construct. e Flow cytometric analysis of surface GRP78 under thapsigargin treatment or serum starvation in MDA-MB-231. The percentage of cells brighter than the control midpoint are given from one of two independent experiments. f Immunofluorescent detection (prior to fixation) of surface GRP78 in 2-deoxyglucose (2-DG)- or thapsigargin-treated MDA-MB-231 and MDA-MB-468 cells. Representative images from one of three independent experiments are shown. Scale bar = 50 μm