Fig. 7

Presence of tumor-reactive immune responses prior to tumor development results in tumor rejection and prevents distant tumor dormancy in FVB mice. Female FVB mice (8–10 weeks old) were challenged with MMC, R-Ctrl, or R-FAC/AIT tumor cell lines in the mammary region (3 million cells/mouse). Naïve mice served as control. a Tumor growth in the mammary region was measured using a digital caliper. b–e Gated FVS−CD3+ T cells were analyzed for CD4+ or CD8+ T cells in the lungs (b, c) or in the liver (d, e). Gated FVS−CD3+CD4+ or CD8+ T cells were analyzed for T effector cells (Te, CD44+CD62L−), T effector memory cells (Tem, CD44+CD62L^low), T central memory cells (Tcm, CD44+CD62L^high), or T naïve cells (Tn, CD44−CD62L+) in the lungs (b, c) or in the liver (d, e)