Fig. 6

T cells predominated with Tem phenotypes inhibit the recurrence of dormant tumor cells, ex vivo. Lungs collected from FVBN202 transgenic mice who had received FAC chemotherapy were cultured alone (medium) or with tumor-reactive autologous lymphocytes (+ T cells, 4 × 10⁶ cells) 2 days after the recovery of dormant cells from the lungs in the presence of 40 unit/ml IL-2 (3 times during the culture as needed) and 20 ng/ml IL-7 (on days 5 and 9 of culture). a Pictures of dormant cells taken at × 20 magnification on day 12 of the co-culture. Arrows show dormant tumor cells surrounded by T cells. Tumor cell apoptosis and Ki67⁻ dormant tumor cells were analyzed on gated CD45−neu+ viable cells. b Gated FVS− viable T cells were analyzed for CD4+ or CD8+ T effector cells (Te, CD44+CD62L−), T effector memory cells (Tem, CD44+CD62L^low), T central memory cells (Tcm, CD44+CD62L^high), or T naïve cells (Tn, CD44−CD62L+)