Fig. 6

Sunitinib-induced senescent ECs recruited cancer-associated inflammatory myeloid cells. a, b Balb/c female mice were pre-treated with sunitinib (100 mg/kg/day) for 2 weeks, followed by inoculation with 1 × 10⁶ transfected 4T1 cells. The monocyte population (gating strategy: Ly6C⁺/CD11b⁺) in the lung, blood, and spleen were quantified by flow cytometry. Data were presented as columns with mean ± SEM (n = 3). c The lung samples were immunostained for CD11b, F4/80, and Ly6G (marked by pseudo-color using ImagePro Plus). d The expression score of CD11b, F4/80, and Ly6G was quantified as described in the “Materials and methods” section. e–h The macrophage (F4/80⁺/CD11b⁺) and neutrophil (Ly6G⁺/CD11b⁺) population in the lung, blood, and spleen were quantified by flow cytometry, and data were presented as columns with mean ± SEM (n = 3). i The infiltrating macrophages were sorted using flow cytometry and subjected to semi-quantitative RT-PCR to detect the level of CD36, CD163, PD-1, IL10, CXCL10, and CCL2. Significance was assessed by unpaired t test with Welch’s correction