Fig. 2

Sunitinib induced a senescence-like EC phenotype in vivo. a Balb/c female (6–8 weeks, 20–25 g, housed in SPF conditions, n = 5) mice were treated with sunitinib (100 mg/kg/day, intragastric administration) for 2 weeks (14 doses total) and inoculated with 1 × 10⁶ 4T1 cells. b The representative images showed the positive staining of SA-β-GAL (marked by pseudo-color using the software ImagePro Plus) in the lungs from the 4T1 tumor-bearing mice. c The scores of SA-β-GAL expression were quantified using 25 randomly chosen fields from 5 mice of each group as described in the “Materials and methods” section. d The lungs from 4T1 tumor-bearing mice were stained for P16, P53, and P57 (marked by pseudo-color using the software ImagePro Plus). e The expression scores of P16, P53, and P57 were quantified using 25 randomly chosen fields from 5 mice of each group. Data were presented as columns with mean ± SEM. Significance was assessed by unpaired t test with Welch’s correction