Fig. 1

Sunitinib induced a senescence-like phenotype in cultured ECs. a HUVEC viability was measured after the treatment of sunitinib. b HUVECs were stained for SA-β-GAL (green) after the treatment of sunitinib. c The score of SA-β-GAL expression was quantified using 10 randomly chosen fields from two experiments as described in the “Materials and methods” section. The data were presented as columns with mean ± SEM. Significance was assessed by unpaired t test with Welch’s correction. d HUVECs were treated with sunitinib and then subjected to WB assay. The densities of the bands for P16, P53, and P57 (referred to β-actin) were presented relative to that of the control (means ± SD, n = 3; the mean level in the control group was set to 1.0)