Skip to main content
Fig. 3 | Breast Cancer Research

Fig. 3

From: Identification and targeting of selective vulnerability rendered by tamoxifen resistance

Fig. 3

Antitumor activity of RITA, AF, and ONC-1 is SULT1A1-dependent. a The dot plot representation of genome-wide shRNA screen in MCF7 cells after DMSO and RITA treatment. Each dot represents a single shRNA. Black dots: shRNA against SULT1A1; red dots: shRNA against SULT1A2 genes; gray dots the rest of shRNAs. Each treatment was performed in triplicate and results for SULT1A1 and SULT1A2 had p 0.05. b Quercetin (10 μM) rescued growth suppression of HCT116 cells by RITA (1 μM), AF (3 μM), or ONC-1 (6 μM) as detected in the colony formation assay (72 h). Shown is crystal violet image. c, d Deletion of SULT1A1 conferred resistance to the three compounds. Shown is cell proliferation assay in SULT1A1-proficient parental cells MCF7 (c) and HCT116 (d), indicated in black line, and the SULT1A1 knockout clones #1 (red line) and #2 (green line), subjected to indicated concentrations of compounds. e RITA (left), AF (middle), and ONC-1 (right) suppressed the growth of HCT116 (WT) cells in a dose-dependent manner, but did not affect SULT1A1 KO clones, as assessed by a colony formation assay (as in d). f Overexpression of SULT1A1-Myc-DDK in A375 and SJSA cells detected using immunoblot. g SULT1A1 expression confers sensitivity to RITA, AF, and ONC-1 in low SULT1A1-expressing cells A375 (upper panel) and SJSA (lower panel). Control vector-transduced cells are indicated by a black line, and SULT1A1-transduced cells by a red line. h Sensitivity of SULT1A1-transduced cells to RITA, AF, and Onc-1 as assessed in colony formation assay. The values in c, d, and g represent mean ± SD of a triplicate (*p  0.05 and ***p  0.001, one-way ANOVA with Bonferroni’s multiple comparison test)

Back to article page