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Fig. 3 | Breast Cancer Research

Fig. 3

From: Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression

Fig. 3

a Amount of E2 in the supernatant of MCF7/PGRMC1 cells compared to the empty vector control after 48 h, detected with ELISA. **p ≤ 0.01. b NFI (blank-corrected mean fluorescence intensity) ratio of protein expression of ERα, Her2, PR, c-Myc, and c-Fos analyzed by RPPA. Protein expression was normalized to MCF7/EVC and protein expression measured in MCF7/EVC cells was set to 1. Up-/downregulation of protein expression in MCF7/PGRMC1 cells were calculated. *p ≤ 0.05 (Student’s t test, n = 3). c Western blot analysis of ERα, Her2, and c-Myc protein levels in MCF7/EVC and MCF7/PGRMC1 cells. Representative picture of 3 independent analyses. d qRT-PCR analysis of ESR1, TFF1, HER2, CCND1, Myc, and PGR mRNA expression in MCF7/EVC and MCF7/PGRMC1 cells, MDA-MB-231/EVC and MDA-MB-231/PGRMC1 cells. *p ≤ 0.05, ***p ≤ 0.001 (Student’s t test, n = 3). e qRT-PCR analysis of PGRMC1, ESR1, HER2, and TFF1 mRNA expression in MCF7 siCtrl and MCF7 siPGRMC1 cells. *p ≤ 0.05, **p < 0.01, ****p ≤ 0.0001 (Student’s t test, n = 3). f Western blot analysis of ERα and Her2 protein levels in MCF7 siCtrl and MCF7 siPGRMC1 cells. Representative blot from 3 independent analyses. g Quantification of HER2 protein in membranes of unpermeabilized MCF7/EVC and MCF7/ PGRMC1 cells, MDA-MB-231/EVC and MDA-MB-231/PGRMC1 cells, and MCF7 siCtrl and MCF7 siPGRMC1 cells (h) via flow cytometry. *p ≤ 0.05 (Student’s t test, n = 3)

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