Fig. 2

Distant recurrence in luminal breast cancer is not associated with strong modulation of immune infiltration. Boxplots showing total count of stained cells per mm² for lymphocytes on HES sections (a), B lymphocytes using CD20 marker (b), CD8⁺ T lymphocytes (c), CD4⁺ T lymphocytes (e), FOXP3⁺ T lymphocytes (f), Th1 T lymphocytes using Tbet marker (g), Th17 T lymphocytes using IL17 marker (h), TLS count on HES sections (i), dendritic cells using DC Lamp marker (j), macrophages using CD163 marker (k), PD-L1⁺ immune cells (l), and PD-1⁺ lymphocytes (m) according to the recurrence status and the molecular subtype. N = 104 patients (52 controls and 52 cases) for all immune analyses except for PD-1 and PD-L1 markers (N = 44, 22 controls, 22 cases). d Representative views of CD4 + T lymphocytes immunostaining in either controls (left) and cases (right) (scale bar = 100 μm for larger views and 50 μm for inserts). p values are from Wilcoxon tests. The color code for boxplots is indicated at the top right of the figure. It depicts the BC subtype assessed by Prosigna™ test. Luminal A BC are in light blue (controls, N = 37) and light red (cases, N = 27). Luminal B BC are in dark blue (controls, N = 11) and dark red (cases, N = 19). HER2-enriched BC are in light green (controls, N = 2) and dark green (cases, N = 2). The basal-like BC is in yellow. BC without result for Prosigna™ test are represented in gray (2 controls and 3 cases)