Fig. 6

Inhibition of FAK and mTOR leads to apoptosis of the Wnt tumor cells and human basal-like breast cancer cells. a Percentage of Annexin V-positive iCtrl-Wnt cells treated with increasing doses of PF271 and PP242 for 48 h n = 6, duplicates from three independent repeats. b Dose response curves from Alamar Blue assay of iCtrl-Wnt cells treated with PF271 and PP242 for 72 h, n = 9, triplicates from three independent repeats. c Immunoblot showing inhibition of FAK and mTOR pathway in iCtrl-Wnt cells upon treatment with PF271(5uM) and PP242 (2.5uM). d Quantification of immunoblot in c. e Dose response curves of MDA-MB-231, CAL-85-1, and HCC1806 cells treated with PF271 for 72 h. n = 9, duplicates from three independent repeats. f Dose response curves of MDA-MB-231, CAL-85-1, and HCC1806 cells treated with PP242 for 72 h. n = 9, duplicates from three independent repeats. All statistical tests include ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, **p ≤ 0.01, ***p < 0.001, ****p ≤ 0.0001