Fig. 5

Transcriptomic analysis indicate mTORC1 pathway is affected upon loss of FAK. a Heat map from RNA sequencing data showing the expression levels of top genes (p < 0.01) that are changed in triplicates of iCtrl vs iKO tumor cells (red—high, blue—low). b Gene set enrichment analysis plot (GSEA) showing ribosome gene signature, mTORC1 signaling gene signature, G2M checkpoint, and E2F target gene signature to be enriched in iCtrl cells, FDR < 0.1. c Immunoblot of FAK, AKT, and mTOR substrates and their phosphorylation in control FAKf/f; MMTV-Wnt1 tumor cells (Wntf/f) treated with 4OHT, iCtrl-Wnt, and iKO-Wnt cells that had been starved for 2 h in serum free media. d Quantification of immunoblot in c, one-way ANOVA, *p ≤ 0.05, **p ≤ 0.01, ***p < 0.001