Fig. 4

FAK ablation sensitizes Wnt1-driven tumor cell to ER stress-induced cell death and reduces their activities in migration and sphere formation. a Immunoblot showing level of FAK in iCtrl-Wnt and iKO-Wnt cells. b, c. Bar graph quantification from AnnexinV-PI flow cytometry of iCtrl-Wnt and iKO-Wnt cells after 48 h of no treatment (b) and serum starvation or amino-acid starvation (c), n = 6. Triplicates from two independent experiments. d, e Dose response curves from AnnexinV-PI flow cytometry of iCtrl-Wnt and iKO-Wnt cells treated for 48 h with thapsigargin (d) or tunicamycin (e). n = 6. Duplicates from three independent experiments. Student’s t test, *p ≤ 0.05, **p ≤ 0.01. f Representative image of scratch-wound assay of iCtrl-Wnt and iKO-Wnt cells at 0 and 8 h and corresponding quantification of percentage of wound closure, n = 6, Student’s t test, p = 0.003. g Representative image of iCtrl-Wnt and iKO-Wnt cells migrated in Matrigel-coated transwell assay and corresponding quantification of cells invaded, n = 6, Student’s t test, p = 0.002). h Bar graph representation of number of spheres formed per 1000 iCtrl-Wnt and iKO-Wnt cells under no attachment condition, n = 24, 12 wells from two independent repeats, Student’s t test, p < 0.01