Fig. 2

FAK deletion suppresses tumor growth and progression in MMTV-Wnt1-driven basal-like mammary tumors. a Tumor-free survival curves of Ctrl-Wnt1 (n = 94), cKO-Wnt1 (n = 37), and cKD-Wnt1 (n = 31) mice. b Immunoblots showing levels of phosphorylated FAK, total FAK, and GAPDH in Ctrl-Wnt1, cKD-Wnt1, and cKO-Wnt1 tumors (n = 3 for each sample). c Immunohistochemistry staining of FAK in Ctrl-Wnt1, cKD-Wnt1, and cKO-Wnt1 tumor sections. d Whole mounts of mammary glands from 5-week-old wildtype, Ctrl-Wnt1, cKD-Wnt1, and cKO-Wnt1 females. e Tumor growth curves of Ctrl-Wnt1(n = 22), cKO-Wnt1 (n = 10), and cKD-Wnt1 (n = 22) mice. Two-way ANOVA followed by Tukey’s multiple comparisons test, **p ≤ 0.01, ****p ≤ 0.0001. f Bar chart showing number of mice (out of n = 21 for each genotype) with various number of metastatic nodules in their lungs quantified from H&E-stained lung sections prepared when mice have similar primary tumor size, chi-square test, p (Ctrl-Wnt1 vs cKD-Wnt1) = 0.02, p (Ctrl-Wnt1 vs cKO-Wnt1) = 0.03