Skip to main content
Fig. 1 | Breast Cancer Research

Fig. 1

From: Clinical and functional significance of tumor/stromal ATR expression in breast cancer patients

Fig. 1

Breast cancer cells downregulate breast stromal fibroblast ATR in an IL-6/STAT3/AUF1-dependent manner. a FFPE tissues isolated from tumors as well as histologically normal tissues (HNT) were immunostained using anti-ATR antibody. Arrows indicate stromal fibroblasts. Scale bar = 50 μm. b TCF-64 cells were treated either with SFM (control) or with SFM containing IL-6 (3.5 mng/mL) or with SFCM obtained from MCF-10A (10A) and MDA-MB-231 (MDA). In addition, TCF-64 cells were exposed to SFCM obtained from MDA-MB-231 cells wherein IL-6 was knocked down using specific siRNA (MDA-si). Whole cell lysates were prepared and used for immunoblotting analysis. c TCF-64 cells were treated as indicated, and then total RNA was purified and used for qRT-PCR. Error bars represent mean ± SD for three different experiments and *P < 3.3.10− 15. d TCF-64 cells were transfected with STAT3-siRNA or a scrambled sequence (Ctrl), and then were treated as indicated and cell lysates were prepared and used for immunoblotting analysis using antibodies against the indicated proteins. GAPDH was used as internal control. e TCF-64 cells were transfected with either p37AUF1ORF or an empty vector (Ctrl), and then cell lysates were prepared and used for immunoblotting analysis. f TCF-64 cells were transfected with AUF1 specific siRNA or with a scrambled sequence (Ctrl), and then both cells were treated as indicated. Cell lysates were prepared and used for immunoblotting analysis

Back to article page