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Fig. 4 | Breast Cancer Research

Fig. 4

From: In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness

Fig. 4

BPA exposure increases the number of collagen fibers and alters the structure of the collagen fibers in mammary glands of adult female mice. a Representative images of second harmonic generation imaging and corresponding picrosirius red staining surrounding mammary ducts in 12-week-old mice. b Quantification of the number of fibers measured by second harmonic generation imaging is depicted. c Histogram of collagen fiber length, quantified using the CT-Fire software. Bin size = 2 μm. d Histogram of collagen fiber width, quantified using the CT-Fire software. Bin size = 0.1 μm. Three glands from oil-treated mice and 4 glands from BPA-treated mice were imaged, and 3 images were taken per tissue section. Overall, 467 collagen fibers in oil-treated mice and 1256 collagen fibers in BPA-treated mice were analyzed, from a minimum of 3 separate litters. *p < 0.05

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Breast Cancer Research

ISSN: 1465-542X

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