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Fig. 3 | Breast Cancer Research

Fig. 3

From: In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness

Fig. 3

Collagen gene expression is altered in mice exposed in utero to BPA. a Mammary glands from 4-week-old mice exposed to BPA or oil in utero were stained with picrosirius red to visualize collagen. Total collagen staining was quantified by the area of red staining in images above the threshold (right graph) and graphed as mean ± SD. A representative brightfield image and threshold image used for quantification are depicted. Area in red of threshold image represents the area quantified. Selected images represent the data point closest to the mean for that sample set. N = 7 for oil and N = 5 for BPA, from a minimum of 3 separate litters. b Mammary glands from 12-week-old mice quantified and graphed as mean ± SD as in a for total area of the mammary gland stained for collagen. N = 11 for oil and N = 13 for BPA, from a minimum of 3 separate litters. c Hydroxyproline concentration was measured in mammary glands from 12-week-old mice exposed to BPA or oil in utero. N = 10 for oil and N = 9 for BPA, from a minimum of 3 separate litters. *p < 0.05; **p < 0.01

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