Table 1 Antibodies used in this study
From: Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment
Antibody | Supplier | Concentration |
|---|---|---|
Endomucin V.7C7, rat monoclonal | Santa Cruz, sc-65495 | 1:100 |
Osterix A-13 rabbit polyclonal | Santa Cruz, sc-22536 | 1:200 |
Alexa Fluor 546 goat-anti rat | Life Technologies, A11081 | 1:400 |
Alexa Fluor 546 donkey-anti rabbit | Life Technologies, A10040 | 1:400 |
Tgif1, rabbit monoclonal | Abcam, ab52955 | 1:500 |
Erk1/2 | Cell Signaling, #9107 | 1:1000 |
Phosphorylated Erk1/2 (Thr202/Tyr204) | Cell Signaling, #4379 | 1:1000 |
AKT | Cell Signaling, #4691 | 1:1000 |
Phosphorylated AKT (Ser473) | Cell Signaling, #4060 | 1:1000 |
Actin, mouse monoclonal | Abcam, MAB1501 | 1:1000 |
Peroxidase-labeled anti-mouse | Promega, W402B | 1:10,000 |
Peroxidase-labeled anti-rabbit | Promega, W401B | 1:10,000 |