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Fig. 5 | Breast Cancer Research

Fig. 5

From: Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment

Fig. 5

Tgif1 supports breast cancer cell migration by suppressing Sema3E expression in osteoblasts. a RNA sequencing-based fold expression of target genes in Tgif1−/− osteoblasts compared to osteoblasts from Tgif1+/+ control littermates. The number of predicted Tgif binding sites on the 3-kb proximal promoter of upregulated transcripts is indicated in the right column. b Semaphorin 3E (Sema3E) mRNA expression in Tgif1−/− and Tgif1+/+ osteoblasts. c MDA-MB-231 breast cancer migration towards increasing concentrations of recombinant Sema3E (0–500 ng/ml). d Migration of 4T1 breast cancer cells towards control medium and medium conditioned by Tgif1+/+ osteoblasts transfected with scrambled control siRNA (Scr) and Tgif1−/− osteoblasts transfected with Scr or siRNA against Sema3E (siSema3E), n = 6 independent experiments. e Schematic presentation of the positive role of Tgif1 in osteoblasts in supporting breast cancer cell migration and metastatic growth. Data are presented as mean ± SEM. Two-tailed Student’s t test was used to compare two groups (b), and ANOVA followed by Tukey’s post hoc analysis was used to compare more than two groups (c, d); *p < 0.05, ***p < 0.001, ****p < 0.0001

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