Fig. 3

Metastatic breast cancer burden is reduced in a Tgif1-deficient bone microenvironment in vivo.a, b Bioluminescence imaging and prevalence of bone metastases 7 days after intracardiac injection (n = 44–46 long bones/group). c Bioluminescence signal quantification shown as average radiance (p/s/cm²/sr) in Tgif1^+/+ and Tgif1^−/− mice (n ≥ 20 mice/group). d–f Representative images of hematoxylin and eosin-stained femurs (d) and quantification of tumor volume (tumor area/tissue area) (e) and trabecular bone volume (BV/TV; bone volume/tissue volume) (f) 9 days after tumor cell injection (n = 4–7 mice/group). Scale bar indicates 1 mm. Data are presented as mean ± SEM. Two groups were compared using two-tailed Student’s t test (b, c, f) or Welch’s t test (e); *p < 0.05, **p < 0.01