Fig. 5

MiR-7/miR-92b targets slug to decrease ESA expression. a Representation of the human ESA promoter showing slug binding at two sites. b PCR-ChIP assay results. c RT-qPCR analysis of miR-92b expression in Lenti-miR-7-infected cells. d RT-qPCR analysis of miR-7 expression after silencing XIST. e RT-qPCR analysis of miR-92b expression after silencing XIST. f Putative miR-7 wild-type and mutated binding sites in slug. g Luciferase reporter activity in MDA-MB-231 and LD cells. h Putative miR-92b wild-type and mutated binding sites in Slug. i Luciferase reporter activity in MDA-MB-231 and LD cells. j Western blot analysis of Slug and ESA expression regulated by miR-7. k Western blot analysis of Slug and ESA expression regulated by miR-92b