Fig. 5

Anillin regulates cell-ECM adhesion and organization of the actin cytoskeleton. a Control and anillin-depleted MDA-MB-231 cells, as well as control and anillin-overexpressing MCF10AneoT cells, were plated on collagen I-coated dishes, and the adherent cell number was determined after 1 h of plating. b,c Control and anillin-depleted MDA-231 cells were fixed and fluorescently labeled for either a FA marker, phosphorylated (P) paxillin (b), or filamentous (F) actin (c). Arrows point on FA localization at the edges of control MDA-231 cells (b), or show the assembly of basal stress fibers in anillin-depleted cells (c). Arrowhead highlights FA located throughout the entire basal surface of anillin-depleted cells (b). Data is presented as mean ± SE (n = 3); ***p < 0.001. Scale bars, 20 μm