Method | Approach | Description | Overall immune score | Cell populations |
---|---|---|---|---|
H&E whole slide (WS) | Microscopy based | Pathology TIL scores | TIL scores | NA |
IHC whole slide (WS) | Microscopy based | Pathology scores with IHC | Summed lymphocyte fractions | Yes |
Whole slide digital (digWS) | Microscopy based | Visiopharm digital scores IHC | NA | Yes |
H&E tissue microarray (TMA) | Microscopy based | Pathology TIL scores | TIL scores | NA |
IHC tissue microarray (TMA) | Microscopy based | Pathology scores IHC | Summed lymphocyte fractions | Yes |
TMA digital (digTMA) | Microscopy based | Visiopharm digital scores IHC | Summed lymphocyte fractions | Yes |
Absolute CIBERSORT (aCBS) [20] | Deconvolution | Cell fractions, absolute mode used | Summed lymphocyte fractions | Yes |
quanTIseq, lsfit (qSEQ) [21] | Deconvolution | Cell fractions, absolute | Summed lymphocyte fractions | Yes |
MCP-counter (MCP) [22] | Gene marker | Arbitrary units | NA | Yes |
xCell [23] | Gene marker | Arbitrary units | NA | Yes |
EPIC [24] | Deconvolution | Cell fractions, absolute | Summed lymphocyte fractions | Yes |
MethylCIBERSORT (metCBS) [25] | Deconvolution | Cell fractions, absolute | Summed lymphocyte fractions | Yes |
TIL rna score (TILrna) [26] | Gene marker | TIL associated gene signature | TIL signature | NA |
meTIL [27] | Gene marker | TIL methylation profile | TIL signature | NA |
Cell signatures Davoli et al. [28] | Gene marker | Computed gene signature | NA | Yes |
Cell signatures Danaher et al. [29] | Gene marker | Computed gene signature | NA | Yes |