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Fig. 3 | Breast Cancer Research

Fig. 3

From: Characterization of organoid cultured human breast cancer

Fig. 3

Tumor-derived primary organoid cultures consist of normal-like and cancer-like organoids. a Representative fluorescence micrographs of cryostat sections from breast cancer biopsies in situ (top) and corresponding representative tumor-derived organoids (bottom) stained for either K19-AF568, K14-AF488, and DAPI (left) or Muc1-AF568, integrin β4-AF488, and DAPI (right). b Quantification of immunostaining of in situ profiles and organoids. (Top) While in situ K14+ cells are relatively rare and almost always part of non-malignant profiles, they dominate in derived organoid cultures. The opposite relation is seen as far as K19+ only cells are concerned. Data are presented as the mean percentage ± SD of the areas positive for K14 or K19 in situ normalized by nuclear staining or the mean percentage ± SD of organoid numbers positive for K14 or K19. (Bottom) Comparison of the numbers of organoids positive for integrin β4 (ITGB4) vs Muc1. Data are presented as the mean percentage ± SD of the organoid numbers. Five independent tumor samples are examined for the analysis, and indicated p values are tested by t test

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