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Fig. 1 | Breast Cancer Research

Fig. 1

From: Characterization of organoid cultured human breast cancer

Fig. 1

Organoid cultures derived from luminal and myoepithelial cells give rise to lineage-restricted organoids. (Top) FACS diagram of the sorting strategy leading to isolation of pure luminal epithelial and myoepithelial cell populations using epithelial marker Trop2 and myoepithelial marker CD271. (Middle) Phase-contrast micrographs of representative primary normal breast organoids from luminal epithelial and myoepithelial cells. A total of 79 and 90 organoids derived from luminal and myoepithelial cells were evaluated (n = 4 biopsies). (Bottom) Representative fluorescence micrographs of cryostat sections from luminal- (left) and myoepithelial-derived organoids (right) stained for either K19-AF568, K14-AF488, and DAPI or Muc1-AF568, integrin β4-AF488, and DAPI

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