Fig. 1

Characterization of palbociclib resistance models developed in ESR1 wild-type and ESR1 mutant (D538G and Y537S) backgrounds. CellTiter-Glo assay, colony formation assay, and western blot analysis of cell cycle proteins of a ESR1^wt-Palbo^S and ESR1^wt-Palbo^R cells, b ESR1^mut: D538G-Palbo^S and ESR1^mut: D538G-Palbo^R cells, and c ESR1^mut: Y537S-Palbo^S and ESR1^mut: Y537S-Palbo^R cells, treated with controls and palbociclib at the indicated doses. d Pathway perturbations in ESR1^wt-Palbo^R vs ESR1^wt-Palbo^S cell lines; red bar represents upregulated genes, blue bar represents downregulated genes