Fig. 6

HEXIM1 is required for KDOAM25 actions. TNBC cells were transfected and/or treated with KDOAM25 (500 nM) as indicated for 10 h (for western blot analyses), 24 h (for differentiation assay and cell cycle analyses), or 6 days (for proliferation assay). a Cells stained with Nile Red to determine lipid droplet formation (differentiation marker, upper left panel), processed for western blot analyses of indicated proteins (upper right panel and lower left panel), or processed for flow cytometry analyses of cell cycle (lower right panel). Panel *P < 0.01 relative to DMSO-treated control at the same cell cycle phase based on t test. b Results of MTT assays and western blot analyses of HEXIM1 and GAPDH expression. *P < 0.01 relative to DMSO-treated control shRNA-transfected cells of the same cell line based on t test. c MDA-MB-231 cells were transfected and/or treated with KDOAM25 (500 nM) for 10 h as indicated and processed for Western blot analyses. Figures are representative of at least three experiments