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Fig. 3 | Breast Cancer Research

Fig. 3

From: Inhibition of the histone demethylase, KDM5B, directly induces re-expression of tumor suppressor protein HEXIM1 in cancer cells

Fig. 3

4a1 and inhibitors of the H3K4 demethylase, KDM5B, upregulated H3K4me2 levels on the HEXIM1 promoter. a Left panel: different breast cell lines were processed for western blot analyses of indicated proteins. Right panel: correlation of KDM5B expression in TNBC with probability of relapse-free survival (RFS) as determined using bc-GenExMiner v.4. b MDA-MB-231 cells were transfected and/or treated with DMSO, 4a1 (100 μM), or KDOAM25 (500 nM) for 2 h; ChIP assays were performed with KDM5B antibody, c H3K4me2 antibody, or d H3K4me3 antibody. PCR primers were for the indicated regions of HEXIM1. Representative western blots of KDM5B expression in KDM5B shRNA-transfected cells are shown in the lower panel in d. Figures are representative of at least three experiments. For b and c, *P < 0.01 relative to immunoprecipitated DNA from DMSO-treated cells and amplified using the same primers based on t test. For d, *P < 0.01 relative to DMSO-treated cells and transfected with the same shRNA based on t test

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