Fig. 1
From: Combining RANK/RANKL and ERBB-2 targeting as a novel strategy in ERBB-2-positive breast carcinomas
Detection of RANK and RANKL expression and subcellular localization in BC cells. a TNFRSF11A (RANK-encoding) and TNFSF11 (RANKL-encoding) endogenous mRNA expression in SKBR3, MCF7, MDA-MB-453, and BT-474 BC cells. Data normalization was carried out against the actin housekeeping gene. b Western immunoblotting showing RANK and RANKL protein levels in human BC cell lines. Actin was used as a protein loading control. c, d The presence of RANK and RANKL was confirmed by immunofluorescence staining. Confocal images present also the subcellular localization of RANK and RANKL in BC cells. Nuclei were visualized using DAPI. Scale bars represent 20 μm. Representative images are shown