Fig. 5

No iatrogenic breast cancer in aged mice after ID EtOH injection. a, b Kaplan-Meier curve for tumor-free survival (a) and for overall survival (b) was plotted for non-transgenic FVB animals injected with 70% EtOH in multiple glands. All animals died of natural causes, with the exception of three animals that were euthanized at designated time point for tissue analyses and censored at this time (630 days of age) for tumor-free and overall survival curves. c, d Representative H&E staining of contralateral non-injected or 70% EtOH-injected glands 18 months after ID procedure. Whole gland tissue is shown in c and representative field along each gland is shown at high power magnification in d. d Some residual epithelial structures can be observed only in the upper left quadrant of the left image of the EtOH-injected gland. Size of the scale bar is 2 mm for images in c and 200 μm for images in d