Fig. 3From: Ductal tree ablation by local delivery of ethanol prevents tumor formation in an aggressive mouse model of breast cancerEffects of different ethanol volumes and concentration on epithelial ablation and collateral tissue damage. a H&E staining of representative non-injected and EtOH-injected whole mammary glands 3 days after the ID procedure. Intact, live epithelial structures are outlined in black. b Box-whisker plot (90/10 percentiles) of the number (in integers) of live epithelial structures or luminal areas (in μm2) of these structures 3 days after the injection based on whole-breast tissue cross-sectional analysis of H&E stain slides; at least four glands were quantitated per group. An epithelial structure is defined as a lobular or ductal structure with more than two adjacent live epithelial cells. Asterisks indicate p value of unpaired Welch’s t test of each EtOH-injected group compared to non-injected control animals (*0.01, **< 0.01, ***< 0.001, ****< 0.0001). c H&E staining of representative tissue field of 50% or 70% EtOH-injected glands at different times after ID injectionBack to article page