Fig. 2

Tissue analyses of mammary glands within 7 days after ID injection of 70% ethanol. a Representative H&E staining of contralateral non-injected or 70% EtOH-injected glands 1 day after ID procedure (same tissues are shown at two different magnifications) and of PBS- or 70% EtOH-injected glands 3 days after ID procedure. b Representative multiplex staining of contralateral non-injected and PBS- or 70% EtOH-injected glands 3 days and 7 days after ID injection. After sequential detection of cytokeratin 19 (CK19), α-smooth muscle actin (SMA) and vimentin (Vim), tissues were counterstained with DAPI. Live duct (black arrow in a and white arrow in b) and ductule (black arrowhead in a and white arrow in b) or ablated duct (orange arrow) and ductule (orange arrowhead) are indicated. b.v. denotes the blood vessel with SMA-stained smooth muscle cells. Scale bars indicate 200 μm in image panels at different magnification