Fig. 1From: Ductal tree ablation by local delivery of ethanol prevents tumor formation in an aggressive mouse model of breast cancerFeasibility of ductal tree filling and in vivo imaging of mammary glands with a 70% ethanol-containing solution. a Dual stain on whole-mount preparation of abdominal glands injected with Evans blue-containing solution of PBS or 70% EtOH. Evans blue serves to track injected solution within the lumen of the ductal tree, and carmine alum stains epithelial cells of the ductal tree. b Tantalum-based contrast agent-containing solution in PBS was sequentially injected within 15 min in the left abdominal (#4) and right abdominal gland (#9); 14-min high-resolution microCT scan was acquired 24 h after ID injection and processed for 3D image reconstruction. c Longitudinal 2-min standard microCT scans were acquired from independent animals whose abdominal glands were injected with tantalum-based contrast agent-containing solution of PBS or 70% EtOH. Different angle views and time points of the same representative glands are shown. Voxels with signal intensities from − 500 to 500 Hounsfield units in original CT slices were selected for volume rendition of diffused contrast agent. Scale bars indicate 1 mm in image panels at different magnificationBack to article page